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Optimized reagents and kits based on immobilized papain, pepsin, and ficin proteases designed to selectively cleave IgG and IgM immunoglobulin molecules into Fab, Fab', F(ab')2 and Fc antibody fragments for use in immunodetection and immunoassay procedures.
FragIT Z kit, Digest and Purify up to 5 x 0.5 mg FragIT Z kit, Digest and Purify up to 5 x 0.5 mgFragIT Z kit for creation and isolation of F(ab’)2 and Fc fragments from 5 x 0.5 mg mouse IgG2a. The kit consist of two spin columns – one for fragmentation (FragIT Z column) and one for purification of F(ab’)2 and isolation of Fc fragments.The FragIT Z column is FabRICATOR Z enzyme immobilized on agarose and the second column has affinity ligands selective for Fc fragments.
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